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Nanjing KCI Biotech Inc. 
Address:699-18 XuanWu Avenue, Nanjing, Jiangsu, China
Phone:86-025-85560000-1588 Mail:;;

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Date: 2015-10-16

Dedicated to High Quality & Fast Reliable
Gross Pathology, Histology, Immunohistochemistry Services
      KCI Biotech offers a full range of histology and pathology services including gross pathology, necropsy, autopsy, routine &customized histology, neurohistology, bone histology, histopathology, cancer pathology, tissue microarray, immunohistochemistry (IHC), immunofluorescence (IF, ICC), apoptosis detection (TUNEL Staining), FISH/ISH, whole small animal organ mount H&E, special chemical staining and IHC, whole slide scanning. We also provide hands-on training, consulting, professional and technical support services. With over 30 years combined academic and industrial working experiences of our pathology team, we are confident that KCI Biotech will achieve high quality work and guarantee you complete satisfaction from project design, development to completion. Please feel free to give us a call to talk with one of our scientists for any questions you may have in mind.
Gross pathology, Necropsy and Autopsy
General Histology Service
•       Paraffin embedding §ioning
•       Cell pellet embedding §ioning
•       Multi-tissue embedding §ioning
•       Frozen embedding §ioning
•       Whole organ paraffin embedding §ioning
•       H&E staining
Special Staining Service
•      We offer the following special staining service using our standard protocols: Alcian Blue, Alizarin Red, Bielschowsky, Congo Red, Fontana Masson, Giemsa, Golgi, Gram, Luxol Fast Blue, Masson Trichrome, Methenamine Silver, Microglia, Nissl, Orcein Elastin, Oil Red O, PAS, Prussian Blue, Safranin O, Sirius Red, Toluidine Blue, von Kossa, X-Gal and others.
•      Please contact us if you need us to develop new staining methods to meet your specific needs.

Bone regular and special staining

Chronic skeleton muscle degeneration, regular and special staining

Pathology evaluation of renal damage, regular and special staining

Pathology evaluation of liver disease, regular and special staining


Immunohistochemical Staining Service
•     We offer the following IHC staining service using our standard protocols: target validation, target screening, efficacy confirmation by IHC, antibody screening, clinical therapeutical confirmation for clinical trial and others.
IHC Routine Service
•     We perform routine IHC, IF and ICC staining using our well-established, optimized staining protocols on formalin fixed paraffin embedded tissue sections, fresh frozen sections, cultured cells and whole mount samples.


IHC Double Label Service
•     We also perform double labeling IHC and IF services to localize two proteins simultaneously on the same tissue section.

Apoptosis Detection Service
•    We offer TUNEL staining for the detection of apoptosis in formalin-fixed, paraffin-embedded tissues, cryostat sections, cell suspensions, cytospins, and cell cultures.
•   We offer other IHC/IF staining for detection or screening of apoptosis related biomarkers.



•       We offer FISH/ISH staining for detection or screening of related biomarkers & targets based on routine & customized probes.

Brain-Array Service
•       Embedding of multiple brains in one paraffin block
•       Sectioning at 5um, 10um, 25um and 50um thickness depending on your specific need
•       Perform H&E staining, Nissl staining, special staining and IHC staining
•       Imaging analysis and stereology analysis
Advantage: Allow simultaneously processing, sectioning and staining all experimental and control brains under same condition therefore reducing experimental variability.
 Whole Mount IHC Service
•     We perform routine Whole Mount IHC staining using standard immunofluorescent and immunoperoxidase methods. We can also help to develop project specific staining protocols according to client's requirements.
IHC Validation Service
•     In order to successfully and consistently perform routine IHC staining, it is critical to develop an optimal protocol for each antibody. Our IHC Validation Service is designed to validate an antibody and develop a standard staining protocol for each antibody therefore reaching the maximal signal to noise ratio.

Pathological Evaluation
•    Scoring, reading, analysis and reporting by Board Certified Pathologists.

Histopathology evaluation of acute pancreatitis


•    Cancer pathology
•    We offer all kinds of cancer pathology including sample collection, processing, regular and special staining and IHC staining for the evaluation of tumor growth, necrosis, metastasis, cancer biomarker/target morphology screening, and analysis in formalin-fixed, paraffin-embedded tissues, cryostat sections, cell suspensions, cytospins, cell cultures and human cancers.

•      Image analysis using standard software

•      Stereology analysis for neuroscience research&l,t;,/SPAN>

•      Serial, analysis of pancreatic islets                                 
•      Whole slide scanning and confocal microscopy                      

•      Whole slide scanning and confocal microscopy
Tissue Microarray (TMA) & Cell Line Microarray (CMA) Construction Service
•      TMA & CMA are powerful technology for high throughput analysis of biomarker expression in a large number of tissue (cell line) samples. These samples include human tissues, animal tissues as well as cell lines. Our TMA& CMA Construction Service includes design and creation of customized paraffin recipient blocks, tissue core transfer and insertion, TMA& CMA block sectioning and slide staining.

Tissue bank
We offer different kinds of tissue banks including mouse, rat, monkey and human normal tissue banks, human multiple different kinds of begin and malignant tumor tissue banks.

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